Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction Nucleic Acid Extraction Reagent Kit,Nucleic Acid Extraction Kit,Covid-19 Nucleic Acid Extraction Reagents,Nucleic Acid Test Kits Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
Repair method of diesel engine body damage
The diesel engine block is typically cast from gray cast iron and serves as the foundation of the engine, supporting various internal and external forces during operation. Over time, it can suffer different types of damage, especially under harsh conditions or improper maintenance. Here are the most common forms of damage and their respective repair methods:
1. **Cracking** – This is one of the most frequent issues, particularly in cold weather. If cracks occur, they must be repaired using cold welding techniques to avoid further stress on the structure. The process involves drilling small holes at both ends of the crack, inserting a series of pins along the crack line, and placing steel plates perpendicular to the crack direction. A low-carbon steel electrode is then used to weld the area, ensuring proper edge coverage. After welding, gently tap the area with a small hammer to reduce stress, and use a flame to correct any warping.
2. **Wear in the 205 Bearing Hole (Upper and Lower Balance Shaft)** – This issue requires immediate attention. For minor wear, you can use the bearing's outer ring method. By heating the bearing with an oxyacetylene torch, you can create a slight bulge on the outer surface and file it down to fit into the worn hole. Alternatively, a 2 mm thick iron washer with a 50 mm inner hole can be used. Drill three small holes through the washer and the block, and secure it with flat-head screws. In cases of severe wear, the original hole can be enlarged, and a bushing inserted. Three small holes are then drilled at the joint between the bushing and the block, and secured with screws.
3. **Cylinder Head Bolt Hole Damage (Strip or Break)** – This often happens due to excessive torque when tightening the cylinder head bolts. If only a few threads are damaged, the hole can be re-tapped and a new bolt matched accordingly. However, if the hole is completely stripped or broken, it cannot be repaired and must be replaced.
4. **Pitting or Cratering** – This type of damage usually occurs due to corrosion or mechanical stress. To fix this, first use a steel cone to enlarge the affected area, then fill it with aluminum wire or spot weld it in place. This helps restore structural integrity and prevent further deterioration.
Proper maintenance and careful handling during repairs are essential to ensure the longevity of the diesel engine block. Regular inspections can help identify early signs of damage before they become critical.