Golden Needle Bag Planting

In recent years, the cultivation of Flammulina velutipes has experienced significant growth. As production increases, market competition has intensified, leading to a focus on achieving both quality and efficiency. While various factors influence the quality of this mushroom, the key to obtaining high yield and superior quality lies in the bud-shooting technique after the mycelial maturation of the cultivation bag. The budding methods for Flammulina include direct growth, regeneration, and the use of fleas. (a) Once the cultivation bag is physiologically mature, it is placed on a bed frame at 14°C (white variety) or 16°C (yellow variety). The tampon and loop are removed, and weakly scattered light is applied to increase humidity. This encourages the formation of mushroom buds. After a certain number of buds appear, the bag’s cover is opened, and newspaper is used to moisten the area, promoting further development into mushrooms. This traditional method is simple to manage and easy to implement. However, it often results in uneven bud formation, affecting the appearance of the final product. Additionally, excessive bacterial growth in the cultivation room can lead to poor air circulation, limiting oxygen availability. Only strong buds may develop properly, resulting in irregular sizes and open caps. White varieties may also have more base fuzz, which affects overall quality. Although 2-3 harvests are possible, the long cultivation cycle and increased management costs make this approach less efficient. (b) In the regeneration method, the tampon is not removed after the mycelium matures. The bag is placed on a shelf with weak light and lower temperatures—14°C for white varieties and 16°C for yellow. The bag remains closed until buds form. Needle-shaped buds grow within the material and the tampon, and once they are well-developed, the tampon is removed, and the bag’s mouth is folded to the surface level. The bag is then ventilated for 4–6 days, either naturally or mechanically, to allow the buds to settle or wilt. Afterward, the bags are moved to the cultivation room, covered with a film to stimulate the formation of lateral branches and new buds. This method yields higher quantities and better quality, with fresh mushrooms reaching 400–450 g (yellow) or 230–280 g (white) per bag. However, the process takes 50–55 days, and the mushrooms tend to be larger with more fluff. This method is suitable only for seasonal cultivation and not ideal for year-round or cold-storage production due to high moisture content, making it unsuitable for refrigerated exports. (c) The third method involves removing the old mycelium from the surface layer after the mycelium has fully grown. Due to low temperature and mechanical stimulation, dense mushroom buds form across the entire surface. The cultivation period lasts 65–70 days. This method offers shorter growing times, supports four-season or cold-storage cultivation, and ensures uniform bud development with a neat appearance. Yield ranges from 240–280 g (white) or 320–350 g (yellow), with 85–90% of buds meeting quality standards. However, it requires precise technical control and more manual effort. The process includes careful selection of the cultivar, ensuring thick, white, and healthy mycelium. Cultivation bags should be fully matured before proceeding, with the upper and lower parts of the bag being plump and the middle part sparse. Proper sterilization and handling during the inoculation phase are crucial to avoid contamination and maintain quality. Humidity and temperature must be carefully controlled, with optimal conditions around 12–14°C and 90–95% relative humidity. Non-woven fabric is ideal for maintaining moisture, and regular misting helps keep the environment stable. In cold storage, ventilation must be adjusted based on external temperatures to ensure proper air circulation and prevent excess moisture buildup.

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