Grass carp is favored by consumers because of its delicate meat quality, large individual size, and less intermuscular puncture, but grass carp “four diseases†restrict the breeding efficiency. Long-term production practice has proved that vaccination is currently an effective way to solve the "four diseases" of grass carp. At low temperatures, the metabolic capacity of grass carp is relatively weak. Second-in-age grass carp can get a new dose of immune antigen through the inoculation route. After 5 days to 30 days of low temperature induction, the immunized grass carp fingerlings complete the initial immunization until the water temperature. At 20°C, the immune resistance level of grass carp was rapidly strengthened to achieve the goal of reducing the chance of second-in-age grass carp disease. Select the vaccination should be selected in the water temperature 8 °C ~ 22 °C in the spring to facilitate the prevention of saprophytic disease. Diseased grass carp should not be vaccinated. All kinds of robust and disease-free grass carp fingerlings above 8.3 cm can be immunized. Disinfect fish fingerlings with 0.5% saline before inoculation. The time is controlled from 5 minutes to 10 minutes. In order to facilitate the production operation, the fish species can be anesthetized with an aqueous solution of trichlorfon. Tool selection The inoculum, needle, and dilution vessel must be sterilized with 75% alcohol or boiled with boiling water. For fish species less than 10 cm in size, 4.5 or 5 inoculum needles are generally used. In species of 10.0 cm to 16.7 cm in size, 5.5 or 6 inoculum needles are generally used, and those in sizes greater than 16.7 cm are generally selected for use on the 7th. Inoculate the needle. In order to prevent needles from getting too deep into the body during inoculation, a small plastic tube can be placed over the inoculation needle so that the exposed tip length is slightly longer than the thickness of the fish's abdominal muscles. Before dilution, the vaccine should be diluted 10 times with 0.65% physiological saline before use. Once a bottle of diluted vaccine is used up as much as possible, if it is used up once, the inoculator can be used to extract the required amount. After extraction, it is used for medical use. The tape covers the eye of the bottle mouth. The inoculation site and dose were inoculated at the basal fin base of grass carp, and the needle and fish were pierced into the fish at a 45 degree angle. For fish species less than 10 cm in size, inoculate 0.5 ml of diluted vaccine at each end. Sizes range from 10.0 cm to 16.7 centimeters per species. Inoculate 0.5 ml to 1.0 ml of diluted vaccine to each species. Specifications for species above 16.7 cm. Tail inoculate 1 ml of diluted vaccine.
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