First, from the delivery room to seize, enter the maternal boar must be full body shower disinfection, delivery room with flame disinfection, to ensure that the sow to enter the delivery room has a clean, comfortable environment. Second, prenatal 3 days with a safe mix of 10 ml 1 needle health care, postpartum cefotaxime + Houttuyfos injection intramuscular injection 2 times a day for 3 days. Third, do a good job of piglet production, to prevent umbilical cord inflammation with Ceftiofur according to the instructions to use straight for 3 to 4 days. Fourth, do a good job of sow care, only if the sow is well managed to inconvenience piglets diarrhea grow faster, reasonable to give full price nutrition and digestible feed, increase green feed and juicy feed. On the same day, feed 1/3 of the diet and gradually increase the amount of feed after the third day. If the mother wild boar is found to be milk-free, Chinese herbal prolactin is used: Vaccaria 60 grams, trichosanthin 60 grams, angelica 10 grams, Astragalus 60 grams, 30 grams of silkworm, 40 grams of pass grass, 1 out of pheasant, 50 grams of brown sugar, 50 grams of ginger, 100 grams of black beans, 150 grams of glutinous rice wine boiled with a small amount of feed to feed a female wild boar, fed twice a day. Five, 7-day-old wild piglets start feeding. Under normal circumstances, the number of deaths of piglets before the age of 7 days accounts for about 60% of the total number of births. The main reasons are: the lack of milk of the mother wild boar, piglets can not eat colostrum, so that the resistance decreased, and Causes diarrhea and coughing. In addition ground feces and urine can contaminate the female wild boar nipples. The fundamental measure is to make high-bed production beds and nursery net beds. The dampness and coldness of the pens are one of the reasons that causes piglets to fall. Six, good piglet insulation is the key to prevent diarrhea, cough, so the floor to pad wood plus infrared heat lamp to ensure the animal's spirit is good and active. VII. Strengthen the professional training of breeders: Keepers should carefully observe the color, breathing, mental status, and eating habits. Once they are found to be treated immediately, the cost is the lowest and the effect is the best. The general cure rate is over 95%. Eight, vaccine use is reasonable, usage and dosage are accurate. Piglets were born 3 days old and were given nasal drip nasal drip. After 30 days, 7 piglets were intramuscularly injected and 25 sows were sow. During the weaning period, 200 grams of powdered milk was added to the diet, and two times a day, free intake of food, free drinking water, keeping the room clean and dry, increased brown sugar in the feed, and antibiotics to prevent diarrhea. After 10 days, we went to weaned pig pens. 60 days for pseudorabies and swine fever. Ninth, sows after weaning to breeding 3 days after the change of pregnancy feed, 17 days began to check the situation, 35 days for the second check to ensure that no estrus re-matching, 90 days into the pregnancy barracks and increase feed, 1 week before delivery to the delivery room, There is a transitional period throughout the cycle to ensure that healthy piglets are born to increase the survival rate of piglets and increase their efficiency. Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. .
Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid.
According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution.
Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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