Aconite identification method

Identification method

This product is yellowish white. There are many starch granules, single spherical, oval or oval, diameter 4 ~ 39μm, umbilical point fork-like, herringbone-like or crack-like; complex grain consists of 2 to 4 granules. The wood fiber is light yellow, mostly bundled, with a diameter of 20-30 μm, a wall thickness of about 5 μm, a single pit, and a starch granule in the cell. Bast fiber is nearly colorless, long-spindle-shaped, and mostly scattered in diameters of 15 to 17 μm. The walls are extremely thick and the pores are not obvious. The pitted tracheal catheter is approximately 68 μm in diameter and the bordered pits are arranged closely. The wood ray cell wall is slightly thicker and the pits are denser. Oil cells are oblong and contain brownish secretions.

Take this product powder 3g, add petroleum ether (30 ~ 60 °C) 30ml, shaking, placed overnight, filtered, the filtrate evaporated, the residue was solubilized with ether 1ml, as the test solution. In addition to take aconite control medicine 3g, with the legal system as a reference drug solution. According to the TLC (Appendix VI D) test, 1 μl of each of the above two solutions was pipetted on the same silica gel H-thin plate, using petroleum ether (30-60°C)-ethyl acetate (4:1) as The developer is spread out, taken out, air-dried and sprayed with 1% vanillin sulfuric acid solution. In the chromatogram of the test sample, spots of the same color were observed at the positions corresponding to the chromatogram of the reference drug.

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