Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. .
Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid.
According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution.
Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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Midsummer Meat Dog Breeding and Management
As summer approaches, temperatures gradually rise, and the growth of harmful bacteria and parasites accelerates, making it more likely for meat dogs to suffer from digestive issues, heatstroke, and infectious diseases. Therefore, it's crucial to implement scientific management practices and create a comfortable living environment for them. Here are five key points for managing meat dogs during midsummer:
First, understanding heatstroke in dogs is essential. Unlike humans, dogs have limited sweat glands, mostly located on their tongues and paw pads. To cool down, they rely on panting, which helps dissipate heat through their mouth and tongue. However, when temperatures are too high or sustained, their body temperature can rise rapidly. This leads to increased metabolic activity, further raising body temperature. High body temperature can disrupt the central nervous system, impair breathing, reduce blood circulation, and cause tissue hypoxia, eventually leading to heatstroke or poisoning.
Second, effective cooling measures are necessary. 1. Avoid direct sunlight: While some sun exposure can be beneficial for bone development, excessive exposure can lead to overheating and dry, damaged coats. It’s best to allow dogs to play in shaded areas during early morning or late evening. 2. Keep kennels cool and well-ventilated: The ideal temperature for puppies decreases over time, starting at 29–32°C in the first week, then gradually lowering to 23°C by the sixth week. Adult dogs prefer 19–23°C. Kennels should be built in shady, ventilated areas, with concrete flooring and occasional water sprinkles to lower temperatures. 3. Provide cold water baths: Although dogs don’t sweat much, cold baths help remove excess body heat through convection. They also help clean the skin, detect skin problems, and prevent external parasites. However, avoid bathing a few days before whelping.
Third, maintaining proper humidity is important. High humidity hinders heat dissipation, increasing the risk of heatstroke, while low humidity can cause dry skin, respiratory issues, and irritation. A humidity level between 50–60% is ideal for meat dogs.
Fourth, preventing food poisoning is critical. High summer temperatures can cause feed to spoil quickly. To avoid this: 1. Use fresh and hygienic raw materials, and store them in cool conditions. 2. Follow feeding standards carefully. 3. Cook meat thoroughly and let it cool before feeding. This ensures food safety and improves appetite. 4. Avoid feeding spoiled or fermented food, as it can cause gastrointestinal distress, acidosis, and even bacterial poisoning. Always discard any questionable food.
Fifth, regular disinfection and disease prevention are essential. Maintaining hygiene reduces the spread of pathogens. 1. Clean kennels daily, removing feces and litter, and disinfect with peracetic acid or Weidao solution monthly. 2. Dry hay and bedding before use to kill germs and absorb moisture. 3. Disinfect feeding bowls weekly using benzalkonium bromide or Weidao solution. 4. Disinfect drinking water by adding alum weekly to prevent illness.
By implementing these strategies, you can ensure your meat dogs stay healthy and thrive throughout the hot summer months.